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Protein sequence

NCBI GI number:
(Example: Type in 22209009 to get use the BLink sequences for gi:22209009)
How to find the GI number for your protein



Substitutions to be predicted on (can be left blank)
The positions of the substitutions must be relative to the protein sequence of the gi entered above.

Enter the substutions of interest [format]:
or enter the filename containing substitutions of interest [format]:




Parameters

Select sequences to include:
If you receive a warning that the sequences were not diverse enough for prediction on your substitution, try "all BLAST hits". If that fails, you can submit here and have SIFT find the appropriate number of sequences.

Remove sequences more than percent identical to query
This cleans out any polymorphic alleles (sequences in the database that are nearly identical to your protein but already containing the substitutions of interest). The contaminating sequences may cause the substitution to be predicted as tolerated.