OPTIONAL
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Protein sequence
Name of file containing protein query sequence (fasta format).
Paste in your protein query sequence (fasta format).
Substitutions to be predicted on (can be left blank)
Enter the name of file containing substitutions of interest [format]:
Enter the substitutions of interest [format]:
Parameters
Select database to search:
Warnings for you predictions? Change this option
Median conservation of sequences
(between 0 and 4.32, we recommend between 2.75 and 3.25)
Remove sequences more than percent identical to query
This cleans out any polymorphic alleles (sequences in the database that are nearly identical to your protein but already containing the substitutions of interest). The contaminating sequences may cause the substitution to be predicted as tolerated.